Welcome to the eilslabs

The eilslabs form a joint research group between the division "Theoretical Bioinformatics" at the German Cancer Research Center (dkfz) and the department Bioinformatics and Functional Genomics at the Institute of Pharmacy and Molecular Biotechnology (IPMB) at Heidelberg University. The group is headed by Prof. Roland Eils.

 


 

Using the LOV2 domain as a light-sensitive switch, the eilslabs team around PhD Student Dominik Niopek and group leader Barbara Di Ventura managed to induced spatial control of desired proteins in mammalian cells. The tool entitled LEXY (for light-inducible nuclear export system) combines the LOV2 domain with a nuclear export signal sequence (NES) and allows to trigger the nuclear export of attached proteins using blue light. Function of the tool was shown by the light-controlled regulation of synthetic repressors and the transcriptional activity of human p53 with light.

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Source: Dominik Niopek

Microscopy images of human embryonic kidney cells in culture. The cells were genetically modified to produce a glowing protein, which was linked to the new optogenetic tool (the LOV2-NES hybrid). Cells irradiated with a blue laser beam (blue arrows) show an efficient nuclear export of the protein. Non-irradiated cells (red arrows) show a constitutively nuclear localisation of the protein.

LEXY supplements the previously developed tool named LINuS (light-inducible nuclear localization signal), which allows the light-controlled nuclear import of proteins.

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