GROUP: intelligent imaging

Novel fluorescence microscopy techniques have extended imaging beyond simply descriptive visual inspection in life science. The intelligent imaging group focuses in particular on automation of high content screening in 3D tumor models.

  • Jabs, J., Zickgraf, F.M., Park, J., Wagner, S., Jiang, X., Jechow, K., Kleinheinz, K., Toprak, U.H., Schneider, M.A., Meister, M., Spaich, S., Sutterlin, M., Schlesner, M., Trumpp, A., Sprick, M., Eils, R., & Conrad, C. (2017). Screening drug effects in patient-derived cancer cells links organoid responses to genome alterations. Molecular Systems Biology, 13(11), 955. doi: 10.15252/msb.20177697
    Feed icon 14px Read the news feature
  • Marin, R., Cortez, D., Lamanna, F., Pradeepa, M.M., Leushkin, E., Julien, P., Liechti, A., Halbert, J., Bruning, T., Mossinger, K., Trefzer, T., Conrad, C., Kerver, H.N., Wade, J., Tschopp, P., & Kaessmann, H. (2017). Convergent origination of a Drosophila-like dosage compensation mechanism in a reptile lineage. Genome Research, 27(12), 1974-1987. doi: 10.1101/gr.223727.117
  • Malte Wachsmuth*,Conrad, Christian*, Jutta Bulkescher, Birgit Koch, Robert Mahen, Mayumi Isokane, Rainer Peppekok, and Jan Ellenberg 2015. “High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells." Nature Biotech 33, 384-389. DOI:
  • Conrad, Christian, Annelie Wünsche, Tze Heng Tan, Jutta Bulkescher, Frank Sieckmann, Fatima Verissimo, Arthur Edelstein, et al. 2011. “Micropilot: Automation of Fluorescence Microscopy-based Imaging for Systems Biology.” Nature Methods 8 (3): 246–9. DOI:

Contribution screening microscopy:

  • Joana Almaça, Diana Faria, Marisa Sousa, Inna Uliyakina, Christian Conrad, Lalida Sirianant, et al. 2013. "High-Content siRNA Screen Reveals Global ENaC Regulators and Potential Cystic Fibrosis Therapy Targets." Cell 154 (6): 1390–1400. DOI:

  • James R A Hutchins, Yusuke Toyoda, Björn Hegemann, Ina Poser, Jean-Karim Hériché, Martina M Sykora, Martina Augsburg, et al. 2010. “Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins.” Science 328 (5978): 593–9. DOI:
  • Beate Neumann, Thomas Walter, Jean-Karim Hériché, Jutta Bulkescher, Holger Erfle, Christian Conrad, Phill Rogers, et al. 2010. “Phenotypic Profiling of the Human Genome by Time-lapse Microscopy Reveals Cell Division Genes.” Nature 464 (7289): 721–727. DOI: http://dx.doi.or/10.1038/nature08869

 Further Publications:

  • Christian Conrad, and Daniel W Gerlich. 2010. “Automated Microscopy for High-content RNAi Screening.” The Journal of Cell Biology 188 (4): 453–61. DOI:

  • Christian Conrad, Holger Erfle, Patrick Warnat, Nathalie Daigle, Thomas Lörch, Jan Ellenberg, Rainer Pepperkok, and Roland Eils. 2004. “Automatic Identification of Subcellular Phenotypes on Human Cell Arrays.” Genome Research 14 (6): 1130–6. DOI: