Welcome to the eilslabs

The eilslabs form a joint research group between the division "Theoretical Bioinformatics" at the German Cancer Research Center (dkfz) and the department Bioinformatics and Functional Genomics at the Institute of Pharmacy and Molecular Biotechnology (IPMB) at Heidelberg University. The group is headed by Prof. Roland Eils.

 


Splicing-CompassA novel method to detect differential splicing from high-throughput sequencing data was developed by the Network Modeling group (Moritz Aschoff, Rainer König) and the HUSAR Bioinformatics Lab (Agnes Hotz-Wagenblatt, Karl-Heinz Glatting) and has recently been published in Bioinformatics.

Aberrant splicing events are associated with various diseases and cancer types, making alternative splicing analysis a very promising approach in cancer research. The emergence of next generation RNA sequencing (RNA-seq) provides an exciting new technology to analyse such events on a large scale. The novel analysis approach uses geometric angles between the vectors of exon read counts derived from RNA-seq data. In collaboration with the Department of Pediatric Oncology and Hematology and Center for Molecular Medicine Cologne (Matthias Fischer), neuroblastoma tumour data was analysed with favourable and unfavourable clinical courses. Thereby, several candidate genes could be identified in neuroblastoma patients with yet unknown or already reported functional relevance in tumour biology.

The method is implemented in the software tool "SplicingCompass", which is licensed under the GNU GPL and freely available as a package in the statistical language R at  http://www.ichip.de/software/SplicingCompass.html

Publication:

SplicingCompass: differential splicing detection using RNA-Seq data. Moritz Aschoff; Agnes Hotz-Wagenblatt; Karl-Heinz Glatting; Matthias Fischer; Roland Eils; Rainer König. Bioinformatics 2013; doi: 10.1093/bioinformatics/btt101