GROUP: Synthetic Biology

How do cells achieve the astonishing level of spatial organization that characterizes them? What are the mechanisms involved? We want to answer these questions using the bottom-up approach of synthetic biology, with a special focus on optogenetics.

 


 

Research ArticlesPublications owl

Niopek D , Wehler P, Roensch J, Eils R * an d Di Ventura B * (2016): Optogenetic Control of Nuclear Protein Export. Nat. Commun. 7. doi: 10.1038/ncomms10624 (*co-corresponding authors)  [article PDF]   [get plasmids from Addgene]   [read the news feature]

 

Wehler P, Niopek D , Eils R and Di Ventura B (2016): Optogenetic control of nuclear protein import in living cells using LINuS. Curr Protoc Chem Biol. 8(2):131-45. doi: 10.1002/cpch.4.

 

Niopek D , Benzinger D, Roensch J, Draebing T, Wehler P, Eils R * and Di Ventura B* (2014): Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat. Commun. 5. doi:10.1038/ncomms5404 (*co-corresponding authors)   [article PDF]   [get plasmids from Addgene]   [read the news feature]

 

Beer R*, Herbst K*, Ignatiadis N*, Kats I*, Adlung L*, Meyer H*, Niopek D * et al.; iGEM team Heidelberg (2014): Creating functional engineered variants of the single-module non-ribosomal peptide synthetase IndC by T domain exchange. Mol. Biosyst.10(7):1709-18. doi: 10.1039/C3MB70594C (*equal contribution)     [read the news feature on the publication and the iGEM Grand Prize Award]

 

Senís E, Fatouros C, Große S, Wiedtke ENiopek D , Mueller AK, Börner KGrimm D (2014): CRISPR/Cas9-mediated genome engineering: An adeno-associated viral (AAV) vector toolbox. Biotechnology J. 9(11):1402-12. doi: 10.1002/biot.201400046             

 

Börner K, Niopek D , Cotugno G, Kaldenbach M, Pankert T, Willemsen J, Zhang X, Schürmann N, Mockenhaupt S, Serva A, Hiet MS, Wiedtke E, Castoldi M, Starkuviene V, Erfle H, Gilbert DF, Bartenschlager R, Boutros M, Binder M, Streetz K, Kräusslich HG, Grimm D (2013): Robust RNAi enhancement via  human Argonaute-2 overexpression from plasmids, viral vectors and cell lines. Nucleic Acids Res . 41(21):e199. doi: 10.1093/nar/gkt836   [article PDF]

 

Kienle E, Senís E, Börner K, Niopek D , Wiedtke E, Grosse S, Grimm D (2012): Engineering and evolution of synthetic adeno-associated virus (AAV) gene therapy vectors via DNA family shuffling. J Vis Exp. (62). pii: 3819. doi: 10.3791/3819   [article PDF]

 

Our publications can also be accessed via pubmed.

 

Popular Science Articles and Comments

Damm U, Hopfengaertner B, Niopek D , Bayer P (2013): Are artists and engineers inventing the culture of tomorrow? Futures. 48:55–64

 

Niopek D *, Berrens R*, Mockenhaupt S, Lewis MD, Mueller AK, Grimm D (2011): To go, or not to go, that is the question - six personal reflections on how geographic mobility may affect your career and life. Bioessays. 33(10):728-31. doi: 10.1002/bies.201100083 (*equal contribution)

 

Stoeckl A, Niopek D ,Eils R (2009): E.colizenz zum Töten. Spektrum der Wissenschaft. Ausgabe April 2009 (in german)